Abstract:
Objectives and method: To determine the velocity of glycine absorption in the chronic experiments under
physiological condition with no operation trauma, pain, narcosis and atrophy of the small intestine using and original
method of surgical formation of the functioning fragment of the small intestine with ‘living fistulas’ in the presence of
chime, all gastrointestinal secrets and natural innervations.
Results: The glycine absorption velocity increases during one hour of perfusion. Absolute parameters of
absorptive activity of the small intestine in the chronic experiments in vivo are higher than in the isolated loop of the
rats’ small intestine. We observed no dissolution of perfusate with gastrointestinal fluids in the small intestine
functioning part indicating that absorption of water in this fragment of small intestine prevails.
Conclusions: Formation and perfusion of the functioning fragment of the rats’ small intestine is an adequate
approach to the investigating the activity of the small intestine under physiological condition. It allows detecting the
impact of the regulatory activity of chime (its exogenic and endogenic components).
