Optimization of extraction conditions for separation of flavonoids from the fruits of Maclura pomifera

Abstract

The aim of this work was to optimize the extraction of samples of a fruit pulp with the purpose of increasing the yield of the final product – flavonoids. The methods of extraction are maceration for 30 days under ordinary conditions of temperature and the 30 minutes’ extraction in a water bath with reflux. The 70% ethyl alcohol was used as a solvent in both cases. For the quantitative determination of flavonoids there was used a spectrophotometric method of the research, based on the determination of the optical density of the colored complex of flavonoids with AlCl3. The optical density was measured at a wavelength of 413 nm, the concentration of flavonoids was found using the calibration curve built for routine [3, p.37]. While extraction by steeping the content of flavonoids was determined in the dynamics in 3, 10 and 30 days. All definitions were carried out in three Parallels with subsequent statistical data processing (calculation of averages, deviations from the mean, dispersion and confidence interval) to determine the reproducibility and accuracy of the results.