DEVELOPMENT OF A PEROXIDASE IMMOBILIZATION METHOD IN POLY-N-VINYLCAPROLACTAM

By the peroxidase inclusion in poly-N-vinylcaprolactam (PVC) the immobilized preparation with а high activity was obtained, which allows to use this biocatalyst for the phenol, hydroquinone, resorcinole, pyrocatechol oxidation in concentrations range 25–75 mmol/dm3 in the presence of hydrogen peroxide. The conditions of peroxidase oxidation, bringing to maximal transformation of phenol, were determined: temperature 20–60°C, pH 4.0–9.0, enzyme activity18.0–25.2 U/mg, molar ratio phenol : hydrogen peroxide 1:1(2), time of incubation 0.5–1 h. Investigation of immobilized peroxidase properties: pH-, thermodependence, thermostability, kinematic viscosity, storage, suggests about the modified, stable form of enzyme formation.