The wound water insoluble hydrogel coating with immobilized alkaline protease, with quantitive yield of proteolytic activity within the range of mass ratio matrix: enzyme (1:0,1–1:0,2), stable at storage and g-sterilization, with prolonged action was developed basing on the modified by polysilicic acid sol polyvinyl pyrrolidone.
It was noted the 100%-retaining of proteolytic activity after immobilization, broadening of pH-profile in the field of neutral pH values, increasing of pH- and thermostability of immobilized enzyme. The kinetic parameters of free and immobilized protease were studied.