The analysis of the data of literature and the results of own investigations of hygienic estimation of virulicide action of chlorine dioxide at disinfection of water have been done. As test-virus they have chosen freshly-extracted (June-November, 2007) and adopted to the culture of cells Coxsackie’s –virus strains (serotype B5) and ECHO-virus (serotype 7). For the estimation of cytopathogenic action of viruses they used the cells of human’s larynx carcinoma Hep-2-Coxsackie; cells of a human’s rhabdomyosarcoma RD-virus ECHO. They used dilution 10-5–10-6–10-7, where dilution 10-5–10-6 was identical contamination of sewage with concentration 50 times as much. Decrease of Coxsackie and ECHO-viruses titer minimum for 1 lg TCD50/ml at the influence of chlorine dioxide minimal doses of 1.0 and 1.5 mg/dm3 correspondingly was more effective and stable in all experiments. The use of chloride dioxide as effective virulicide means for disinfection of drinking water and sewage.