Based on isolated Agaricus bisporus fungi tyrosinase, immobilized in poly-N-vinylpyrrolidone, modified by polysilicic acid sol, the biocatalyst was obtained, being suitable for heterogeneous catalysis, with increased stability at elevated temperature conditions. Under conditions developed (pH 6.5, temperature 25 °C, time of transformation — 1 h, phenoloxidase activity 50–1000 U/cm3) the preparation had catalyzed total substrate oxidation in batch reactor during 8 cycles with high level of its transformation retaining during the next 7 cycles.
