Epigenetic changes play important role in tumor transformation of endometrium. One of the pathogenic mechanisms of epigenetic cell transformation may be a methylation of promotor and first exon of gene-supressors of tumor growth.
The aim of the study was a comparative analysis of gene WIF1 methylation in women with hyperproliferative processes of endometrium and endometrial carcinoma. Methylation analysis was done by using COBRA method, which could detect the amount of methylated DNA in tissue sample, unlike methylspecific PCR method. It was established the absense of gene WIF1 methylated DNA as in endometrial hyperplasia, carcinoma and in normal endometrium.